# How To Calculate Ic50 Value In Spss

Step 4: Percentile Calculation 90 th Percentile = Value corresponding to the Index Number (23) = Value at 23 rd place in data set = 89 Hence, the 90 th percentile of the given data set is 89, which is the value at the 90 th percentage of the data set, which is the value at the index number 23 out of the total 25 numbers. It's very popular, it's very powerful, and it's a great way to work with your data for new insights. Calculate the range for your confidence statistics. pdf Michael Hallstone, Ph. Say instead of considering the Clinician rating of Susser Syndrome a gold standard, you wanted to see how well the lab test agreed with the clinician's categorization. 985? If SPSS generated a negative Pearson's r value, we could conclude that when the amount of water increases (our first variable), the participant skin elasticity rating (our second variable) decreases. 312 from "Based on the count values obtained rxy 0. Open the SPSS program, then click the Variable View, in the Name write Gender 2. The simple answer is that you don't have to interpret it on its own, because you have p-value to judge whether this value is significant or not. This will be enough for majority of research done in SPSS. In regular conversation, both words are often used interchangeably. Due to the lengthy calculations, it is best to calculate r with the use of a calculator or statistical software. This is probably an opportune moment to define Weight of Evidence (WOE), which is the log component in information value. In the Value box under New Value group input 6, then click Add tab. Value Paste your data in columns b and c Select the type of mean square and then fit curve by pressing the optimise fit button In the case the fit does not work, in column c, use the graph as guidance to estimate the starting values Change the IC50 and slope values IC50 2. dmy(1,1,2015) and thus create our guessed completion date as a new variable in our dataset. Convert IC50 to pIC50 with a calculation? There is a formula pIC50 = -logIC50 would any one help out in solving this problem. Examples of descriptive statistics include: mean, median, mode, standard deviation, and range. test command. I will focus on the most basic steps of conducting this analysis (I will not address some complex side issues, such as assumptions, power…etc). The cost calculator enables parties to produce an estimate of the likely costs of an ICC Arbitration according to the scales in Appendix III to the Rules. The procedure can be used to fit heteroscedastic probit and logit models. It seems a little counter-intuitive, but the Descriptive Statistics tab does not give you the option to find the mode or the median. but y values are not in a trend. Instructions: Type any number and the solver will compute its absolute value. The Mean or Average. If you are still struggling to calculate d values by using the formula, we have created a Cohen's d calculator. In SPSS, you can use the DATE function to calculate age from birthdate data, but the exact commands depend upon the format of the birthdate. 42 μg/mL) and α -tocopherol (IC50 = 62. Statistics in the complete detailing of data right from the planning of data collection in the form of surveys and experiments. For this purpose there is formula to convert the IC50 value to pIC50 value with representing the logarithmic values. IC50 value is NOT used to calculate dose. To create a CSV file with a text editor, first choose your favorite text editor, such as Notepad or vim, and open a new file. Along with knowing the R-value of a particular insulation, it is also important to calculate the R-value of a total system. The epsilons, which are measures of degree of sphericity, are less than 1. Once you have your data set loaded into SPSS you can stack your data file using the following steps. Often, outliers in a data set can alert statisticians to experimental abnormalities or errors in the. The half maximal inhibitory concentration (IC 50) is a measure of the potency of a substance in inhibiting a specific biological or biochemical function. How to calculate IC 50 value using Excel? ED50V10 (Readme) is an Excel add-in for calculating IC50/EC50 values. It is most commonly used when you have multiple Likert questions in a survey/questionnaire that form a scale and you wish to determine if the scale is reliable. IC50 values of MBIs are time dependent, causing serious problems when aiming at ranking different compounds with respect to their inhibitory potential. Here is a collection of all basic and advanced math statistics calculators online. The Medical Expenditure Panel Survey (MEPS) is a set of large-scale surveys of families and individuals, their medical providers, and employers across the United States. For this, QSAR studies can be used, and have extensively been used (filter through merely related papers). Could you may be able to solve one more of my problem. But when you actually go to fit data to determine these values, there are several complexities and ambiguities. You may see warnings about the file encoding for SPSS save files: it is possible such files contain non-ASCII character data which need re-encoding. The Mean or Average. This "quick start" guide shows you how to carry out a one-sample t-test using SPSS Statistics, as well as interpret and report the results from this test. How to Calculate Cronbach Alpha in SPSS? There’s no better way to measure internal consistency with the software than setting up the Cronbach alpha in SPSS. Several computer programmes (SAS, SPSS,POLO, LeOra etc) accurately calculate LD or LC values but these programmes are not free and require knowledge of using them. Dear Rajeev sir can u tell me How to calculate IC50 value of a compound with known GI50 value? I have only known structure and GI 50 value. I tried linear iterpolation, I am not getting good. School of information science and Engineering, Shandong Normal University, Jinan, China. I read the explanation but still couldn't understand how it works (I'm wondering how to do it by hand because I have a test tomorrow and by hand is the way to go). To correct for this, simply add one participant to the end of the data set in SPSS. The Kaplan-Meier method (Kaplan & Meier, 1958), also known as the "product-limit method", is a nonparametric method used to estimate the probability of survival past given time points (i. In this article, I have explained step-by-step how to log transform data in SPSS. 05, assuming that that is your desired experiment-wise alpha. You can manually calculate the time between date_of_enrollment and bday for some of the cases in the data and then compare the manual calculation to the value SPSS created in the new variable age_at_enrollment. Quick P Value from Pearson (R) Calculator. Package 'ic50' February 20, 2015 Title Standardized high-throughput evaluation of cell-based compound screens Version 1. As long as a case has at least n valid values, the computation will be carried out using just the valid values. This assumes a linear relation. 000 but would round to. SPSS will name the second column for you. Do this by taking the square root of the variance, if it is given. That is, a column is set up for each of the items to which a respondent can answer Yes or No. But what if you want to create a correlation matrix across a range of data sets? To do this, you need to use Excel's. Ideal for marketing research. moderating effects). It can be used to model dose-response, ligand-binding, enzyme kinetics, and growth inhibition. You can manually calculate the time between date_of_enrollment and bday for some of the cases in the data and then compare the manual calculation to the value SPSS created in the new variable age_at_enrollment. One way is. Also, the symbol “ ≈” means approximately equal, with the approximation being more accurate in relative terms for smaller absolute values, as shown in the table below. Add the test variable (Height in this case) into the Test Variable(s): window. 05, assuming that that is your desired experiment-wise alpha. A visual explanation on how to calculate a regression equation using SPSS. DRT may be right. $d'$ Calculator Overview. Also keep in mind that a negative value for b represents an S-shaped curve which increases rather than decreases. Chapter 5 Analysis of variance SPSS –Analysis of variance Data file used: gss. 05, we would conclude that the difference was significant. This Site Might Help You. How Do We Calculate the Standard Deviation From these Two Range Statistics?. Click here to calculate using copy & paste data entry They are different, but not different enough to matter -- like the maple leaves off the tree in my yard, when all I want to do is rake them up. 001 if SPSS reports. In this case, 20/4 = -5 fold. what is the right way of determining IC50 using microsoft excel? I went across several articles that used excel to calculate ic50, and there is one term 'linear interpolation'. A principal component analysis and entropy value calculate method in SPSS for MDLAP model. This command shows you how many cases were classified correctly and incorrectly, using a cutoff point of 50% for the predicted probability. How to calculate and use predicted Y-values in multiple regression December 18, 2014 / Meng Hu / 0 Comments I was planning to publish this article after my paper on the black-white vocabulary gap in the GSS is released, but I have changed my mind. Statistics Tutorials. I just want to know, whether, for all this while i calculate the correct way using excel. SPSS has a very similar interface to Microsoft Excel. As i am regular user for Base SAS,SQL and Macros but i did't come across to Statistical procedures. Numerical values are calculated with precision up. The ANOVA test procedure produces an F-statistic, which is used to calculate the p-value. 2*1e-6) then here i got problem in sol. [email protected] SPSS will either use “listwise deletion” or “pairwise deletion” of the missing values. How to Analyze Ordinal Data in SPSS Using Different Tests. Listwise deletion – SPSS will not include cases (subjects) that have missing values on the variable(s) under analysis. In conclusion, SPSS is a great tool in testing and checking for multicollinearity. what is the right way of determining IC50 using microsoft excel? I went across several articles that used excel to calculate ic50, and there is one term 'linear interpolation'. To illustrate how to do regression analysis in SPSS, we will use two interval variables from the sample data set. 001 if SPSS reports. The red line in that plot show the average values on the Y coordinate (Juridictional Hierarchy, scaled from 1 to 5) for each value of the X. Click on the bottom right corner of that cell and drag down to the bottom of your document, which will automatically calculate the periodic daily returns for each row. This study highlights the factors that investigators need to consider when using software programs to calculate IC 50 values based on the shape of their inhibition curve. 68 SPSS The Wagner Way You also know how to calculate the test statistic chi from PL SC 502 at New York University. Move the variable to the right and check the box “Save standardized values as variables” Close the Statistics Viewer (because we don’t need this) and go back to Data View. There are two types of research in statistics such as quantitative and qualitative data analysis. Sig value. You can calculate the odds ratio using binary logistic regression analysis in SPSS. In today's blog entry, I will walk through the basics of conducting a repeated-measures MANCOVA in SPSS. P value is a statistical measure that helps scientists determine whether or not their hypotheses are correct. Chapter 5 Analysis of variance SPSS –Analysis of variance Data file used: gss. If there is correct way to determine from the concentration vs cell viability data, it would be really nice. Do this by taking the square root of the variance, if it is given. I read the explanation but still couldn't understand how it works (I'm wondering how to do it by hand because I have a test tomorrow and by hand is the way to go). According to the FDA, IC 50 represents the concentration of a drug that is required for 50% inhibition in vitro. To make this alittle more automated, one can write a macro that evaluates the expression. Thanks In advance. When conducting a statistical test, too often people immediately jump to the conclusion that a finding "is statistically significant" or "is not statistically significant. Using the same 2×2 table as you used in Question 2, calculate Kappa. This calculator can be used to find Mean, Median, and Mode. How to calculate a x value from given y value based on the above data. You can calculate the odds ratio using binary logistic regression analysis in SPSS. Choose Curve Fit > General > Sigmoidal. Quick Overview • Probit analysis is a type of regression used to analyze binomial response variables. Now, let's perform the independent t-test in SPSS. IC50 values, in contrast, can vary since they depend on the substrate concentration used in the IC50 determination. Home > IBM Processor Value Unit (PVU) Calculator Processor Value Unit (PVU) licensing for Distributed Software. LOGIT(p) returns the logit of the proportion p: The argument p must be between 0 and 1. How to Read this Output:. A Z-score, also known as a standard score, represents the number of standard deviations (SDs) a data point is away from the average (mean) of the group. Molecular Modeling and Drug Design Laboratory, BCSIR Chittagong. This assumes a linear relation. The group is determined by the stage and period, so in the end there should be a column with the values of the wages in period 1 stage1, period 1 stage 2, period 2 stage1 Unfortunately, I haven't much of a clue on how to program this. This equation is the same as the Dose Response Logistic equation found in the IC50 folder (see below). A Z-score, also known as a standard score, represents the number of standard deviations (SDs) a data point is away from the average (mean) of the group. I ran a (1,0,0) model in ARIMA, resulting in coefficient estimates of 3. Do this for each value, and then add all the values together. Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines. If your analysis implies two variables, e. SPSS has a nice little feature for adding and averaging variables with missing data that many people don't know about. I'm working on predicting the binding of peptides to MHC molecules using assay data from IEDB. SPSS assumes that this value equals 0 unless the user indicates otherwise. Generate a new variable dwell, which categorizes households according to variable using_a: assign 1 to dwell if household has own dwelling and 2 if no. 4nM, 6nM, 8nM, 6nM and 1. Can anybody tell me how to use SPSS software to get the Sensitivity, Specificity, Positive. When you have two groups and at least one or more levels of information to compare, using ANOVA to calculate the data can help you to know if your hypothesis is true or false. If we take the square root of this number, it should match the value of the Pearson correlation we obtain. With increasing drug, this decreases to a value of 0%. As a result of the study acetonic extracts from Boletus edulis was more powerful antioxidant activity with IC50 value of 4. If not, find the variance: Take a value in the sample, subtract it from the sample mean, and square the difference. Paste in X, Y values: Paste in the X and Y values here. Example of a data file in a spreadsheet. I have 4 independent variables. The following examples present three formats for birthdate data: You may have entered the birthdate data using the following commands with American date format (i. I tested the IC50 values of compounds known to inhibit the enzyme in my protocol and have the IC50 values of the previous protocol. If there is correct way to determine from the concentration vs cell viability data, it would be really nice. Learning how to calculate beta is vital, as it is used to measure risk, such as in the examples provided that break down the beta for Apple and Tesla. Create your plot. Odds = p/(1-p) [p = proportional response, i. The type is chosen from the list of types shown here. Or it should read "should never use Excel for IC50 estimation if you can't write a macro that incorporate the four parameter model for nonlinear fitting of a sigmoid dose-response curve. 000), and the number of pairs ( N =9). This will be enough for majority of research done in SPSS. Log transformation. Cronbach's alpha is the most common measure of internal consistency ("reliability"). Do this for each value, and then add all the values together. It was developed by Belgium Statistician Adolphe Quetelet approximately 150 years ago. one column for weight and the other for height how would I go about calculating the P value?. SPSS allows you to complete a number of statistical procedures including: measures of central tendency, measures of variability around the mean, measures of deviation from normality, and information concerning the spread of the distribution. It assumes a value 0 in ANOVA in SPSS when all the category means are equal, indicating that X has no effect on Y. If variables are defined with the. To calculate a weighted percentage, first multiply each item by the percentage it has been allotted, and then add those values together. Emphasis is given to the four-parameter model or sigmoidal model, which is frequently used to calculate EC50 (IC50, DC50, or GI50) values in dose-response experiments such as drug screening and inhibition assays. IC50-to-Ki is a web server that estimates K(i) values from experimentally determined IC(50) values for inhibitors of enzymes and of binding reactions between macromolecules (e. How to Calculate Outliers. Input your data in the left columns, and your results will be shown in the right. For example, a very common situation is a researcher needs to average the values of. How do I calculate in Excel the concentration of a drug that gives half-maximal response? What is the equation to plot a dose-response curve - either for an increased response (EC50) or an. By doing so, SPSS will automatically set up and import designated variable names, variable types, titles, and value labels, meaning that minimal legwork is required from researchers. However, they are not all found in the same place. Entering and manipulating information in the application can be done by using SPSS's proprietary language, which is known as the Syntax command language, or more commonly, as Syntax. 0 to perform one-sample t-tests, independent samples t-tests, and paired samples t-tests. Can anyone give a tutorial for LC50 calculation in SPSS? Can anybody tell me how I can enter my data in SPSS in order to calculate ED50 or IC50? How to calculate LC50 and LD50 value of. This Site Might Help You. Why? The InterpX function finds the corresponding X value for a given Y value; the IC calculation is not necessarily the corresponding X for a Y value of 50. I have a dose response curve consisting of various data points. IC50 values, in contrast, can vary since they depend on the substrate concentration used in the IC50 determination. 988), the two-tailed statistical significance (. Puromycin IC50 - (reply: 1) Best method to calculate IC 50 MTTv/s clonogenic assays - (reply: 4) LDH assay to get GI50? - (reply: 2) IC50 - Is there easy way to calculate IC50 from MTT data (reply: 5) How to make an immortalized cell line/ How to determine IC50? - Protocol for developing a drug resistant cell line? (reply: 6). Within SPSS the data looks like this. This is the same as the algebraic method of rearranging the 4P equation. SPSS does not come out of the box with this functionality, but I have attempted to replicate it utilizing the PRINT command with temporary variables, and wrap it up in a MACRO for easier use. But im given IC50 value of the inhibitor in mol/L. User missing values are values that are invisible while analyzing or editing data. The final shape looks as under. In usual step for calculation of the assay value of ELISA is to draw a standard curve, absorbance on Y-axis against concentration on X-axis, then to estimate assay value from the absorbance of the sample. (2) Calculate one column of means based on all 100 variables, How to replace missing value with mean in. How to Calculate Outliers. School of information science and Engineering, Shandong Normal University, Jinan, China. This p-value calculator is a tool that can help you determine the p-value of a standard normal distribution. The Missing attribute is a place for you to designate certain data values that you want SPSS to ignore when it calculates statistics. Zβis the standard normal z-value for the power of 80%, which is 0. (Notice that adding the linear regression trend line will also add the R-squared value in the margin of the plot. 5, the curve is shallower and the EC90 equals the EC50 times 81. SPSS allows you to complete a number of statistical procedures including: measures of central tendency, measures of variability around the mean, measures of deviation from normality, and information concerning the spread of the distribution. What is the T Score Formula? A t score is one form of a standardized test statistic (the other you'll come across in elementary statistics is the z-score). These descriptive statistics can be calculated using software such as SQCpack. In our example, our Pearson's r value of 0. These tutorials now have their own dedicated home at EZ SPSS. In the Value box under New Value group input 6, then click Add tab. By doing so, SPSS will automatically set up and import designated variable names, variable types, titles, and value labels, meaning that minimal legwork is required from researchers. I hope you know a solution to my problem. School of Management science and Engineering, Shandong Normal University, Jinan, China, HONGGUO WANG. 18 corresponds to 85. Log transformation. Often, outliers in a data set can alert statisticians to experimental abnormalities or errors in the. How to Make a Histogram in SPSS; How to Calculate Mean and Standard Deviation in SPSS. DRT may be right. How to determine an IC50. Like selective serotonin re-uptake inhibitors (SSRIs) drug citalopram inhibits the serot. In this particular example, we will test the claim that the mean height is = 65. 245 for CONSTANT and 0. You can do this by spot-checking some of the rows in your data. The IC50 reported as part of the main results table will be the relative IC50. Sample means are important in that they can give an idea of central tendency-- that is, an idea of the general tendency of a set of numbers. You must take the entire Quiz 2a during one visit to the Exam Commons within these dates. Finally, divide the chart values by a million, so that instead of going from 0 to a million to goes from 0 to one. 23 FAQ-251 How to compute EC50/IC50 in Dose Response fitting. 583> r table product moment 0. Substantive comments on the book will be published elsewhere, so you may want to watch this space for updates; but what I want to do in this post, instead, is share some thoughts regarding research methods and. 5% or a P of. Can anyone tell me about any free software that calculates IC50 values from binding energy or theoretical data? Any help will be heartly appreciated. Make sure you do this. BEGIN DATA. I am grateful that I learnt to calculate the requrired values, in excel. SPSS RND Function. Seems simple enough. It was developed by Belgium Statistician Adolphe Quetelet approximately 150 years ago. SPSS: Analyze: Inferential Statistics Tests About Means Compare Means : One Sample T-Test. To calculate the IC50 value: The inhibitor concentration against the percent activity is plotted ([I]-Activity % graph). Paste in X, Y values: Paste in the X and Y values here. IC50's are bad for you 2 What is enzyme inhibition on the molecular level "Drugs produce their inhibitory action by combining with the enzyme [molecules]. First, you must choose the variable which you are recoding and name the output variable which will hold your new value. Also, IC50 values are dependent on the conditions in which they are measured, so it's not possible to state a simple relationship between them It is possible to calculate the IC50 value of a ligand-receptor complex with any of the Schrödinger software? | Schrödinger. IC50 - Is there easy way to calculate IC50 from MTT data (May/30/2007 ) You should titrate concentrations (\2 dilutions ) near 10-15 points for unknown drug behavior on your cells. Statistical analysis allows you to find patterns, trends and probabilities within your data. Manipulating Variables in SPSS It is likely that you will want to manipulate your variables at some point after entering them. Data can be from Excel or CSV. You can manually calculate the time between date_of_enrollment and bday for some of the cases in the data and then compare the manual calculation to the value SPSS created in the new variable age_at_enrollment. import spss, spssdata to calculate the mean absolute difference in life expectancies around the Re: Calculation of. 001 would still be safe. SPSS descriptive statistics are designed to give you information about the distributions of your variables. display an average for a single variable, the average will be based on the valid values (valid n) for that variable. Putting this into a calculator comes out with a value of 1. A new web-server tool estimates Ki values from experimentally determined IC50 values for inhibitors of enzymes and of binding reactions between macro We use cookies to enhance your experience on our website. This is the same as the algebraic method of rearranging the 4P equation. It is determine for checking antagonist potency. Finally, it's important to understand what happens when the product of the LSD p-value and the number of comparisons exceeds 1. A table is generated. 985 was positive. In order to generate descriptives in SPSS, you first need to open up a data set. Move the variable to the right and check the box “Save standardized values as variables” Close the Statistics Viewer (because we don’t need this) and go back to Data View. While toxicity tests on mice and rats do not always extend to people, the LC50 value is important and is used to be on the safe side when humans work around the material. We want to look up the z-score z*for which the area between -z* and z* is 0. Fitting the ECanything directly using Prism. To calculate Pearson’s r, go to Analyze, Correlate, Bivariate. Calculate the residual variance. Excel refers to R^2 as the correlation coefficient. In the menu bar, navigate to Analyze > Descriptive Statistics > Crosstabs, select the Row and Column as below. Means and standard errors. From SPSS For Dummies, 2nd Edition. 23 FAQ-251 How to compute EC50/IC50 in Dose Response fitting. Computing the Standard Deviation in SPSS The standard deviation is a measure of variability. The answer to each of these questions is regarded, for the purpose of SPSS coding and data entry, as. If there are numerous similar questions in a survey or test that form some sort of scale this kind of an inspection can determine whether the scale is valid or not. Absolute value. Scroll down for the answer. ENTERING THE DATA: For this example, we are using the HSB500 (High School and Beyond) data file. 18 corresponds to 85. In other words, when you have values for all variables in a case except one, you can specify a placeholder for the missing value. Quick Tutorials. The value of 1 was assigned to the Holstein label, and the value of 2 was assigned to the Jersey label. If I decide a one. The Wilcoxon Sign Test in SPSS. I have run an analysis on data using SPSS. In SPSS, you can modify any function that takes a list of variables as arguments using the. I've found a fair number of formulas online which let me convert birthdate into decimal age in years but I can't find something that converts, for instance, 1. How to Calculate Cronbach Alpha in SPSS? There’s no better way to measure internal consistency with the software than setting up the Cronbach alpha in SPSS. LOGIT(p) returns the logit of the proportion p: The argument p must be between 0 and 1. This is the same as the algebraic method of rearranging the 4P equation. 0 to perform one-sample t-tests, independent samples t-tests, and paired samples t-tests. EBSCOhost serves thousands of libraries with premium essays, articles and other content including Tool development for Prediction of pIC50 values from the IC50 values - A pIC50 value calculator. Quick P Value from Z Score Calculator. But this gives only one value from the straight line equation. Linear Regression. A common use of least-squares minimization is curve fitting, where one has a parametrized model function meant to explain some phenomena and wants to adjust the numerical values for the model so that it most closely matches some data. Move the average marks field to the right box for analysis, and then enter 50 as Test Value, click on OK. Putting this into a calculator comes out with a value of 1. Total Views of MMDDL. The critical value will then use a portion of this alpha on each side of the distribution. Calculator gives equation of four-parameter logistic (4PL) curve as well as graph. I have 4 independent variables. You might want to calculate a new. Through statistical analysis using the sample mean, statisticians can calculate items such as standard deviation and variance. P values are used to determine whether the results of their experiment are within the normal range of. n suffix, where n is an integer indicating how many nonmissing values a given case must have. pdf Michael Hallstone, Ph. ZIPENG ZHANG. IC50 (Inhibitory Concentration 50%) is the concentration of drug that is require for 50% inhibition in vitro. Lower limit = 50-17. To make this concrete, consider an alpha of 5%. If we take the square root of this number, it should match the value of the Pearson correlation we obtain. display an average for a single variable, the average will be based on the valid values (valid n) for that variable. Enter your two variables. How to calculate a x value from given y value based on the above data. Change in natural log ≈ percentage change : The natural logarithm and its base number e have some magical properties, which you may remember from calculus (and which you. If the value is Based on Mean Sig. The plots provided are a limited set, for instance you cannot obtain plots with non-standardized fitted values or residual. Using SPSS for OLS Regression Page 6. Use to test the claim that a population mean is equal to a specific value. Easy Bar Chart Creator. The simplest estimate of IC50 is to plot x-y and fit the data with a straight line (linear regression). Anova in SPSS. This equation is the same as the Dose Response Logistic equation found in the IC50 folder (see below). SPSS is a statistics and data analysis program from IBM. 10 END DATA. Put in the reverse, statistics provide an interpretation and summary of data. If there is correct way to determine from the concentration vs cell viability data, it would be really nice. Sample means are important in that they can give an idea of central tendency-- that is, an idea of the general tendency of a set of numbers. School of information science and Engineering, Shandong Normal University, Jinan, China. Cronbach's Alpha (α) using SPSS Statistics Introduction. When using syntax to calculate the difference between two dates, functions are used to process date values. Use the Excel function CORREL to calculate R^2 and validate your answer in Step 6. For example, you would place “ragree” and “recip” in step 1. Definition of IC50 in the Definitions. For example, if I desired an 80% probability of detecting an effect that I expect will be equivalent to r =. I have a problem with SPSS. My problem is that I have no background in statistics, and I can't figure out what the tests are called, or how to calculate them. Use SPSS to calculate Z Score and probability. Absolute IC50. 05, assuming that that is your desired experiment-wise alpha. I'm following a statistics course for the first time and wondering how can I calculate the p-value from the information given in the picture. Given a problem, which test should be used? 2. Each X value must be a substrate concentration and it should be followed by the corresponding experimental measurement of the reaction rate, Y, with an intervening space or text separator.